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KMID : 0363620110320010175
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Journal of Korean Oriental Medicine
2011 Volume.32 No. 1 p.175 ~ p.184
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Effects of Angelicae Pubescentis Radix Water Extract on Immune Property in RAW 264.7 Macrophages
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Lee Jong-Han
Kim Yun-Sang
Lim Eun-Mee
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Abstract
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Objectives: The purpose of this study was to investigate the effects of Angelicae pubescentis Radix water extract (ACE) on immune properties in macrophage cells.
Methods: The cells were divided into two groups: As a control, the first was not treated with ACE, and the other was treated with ACE. Together with the cell viability, productions of nitric oxide (NO) and cytokines such as interleukin (IL)-, IL-6, and tumor necrosis factor (TNF)- by treating of ACE were monitored.
Results: 1. There was no decrease of the cell viability after 24 hr incubation, but a significant decrease after 48 hr incubation with all four concentrations (25, 100, 200, and ) of ACE. 2. A significant increase in the production of NO was observed in the concentrations above of ACE after 24 hr incubation. 3. Further, after 48 hr incubation, the critical concentration of ACE for the increase was reduced to . 4. The production of (IL)- significantly increased with the ACE concentrations of 100 and after 24 hr incubation. 5. The production of IL-6 significantly increased with the ACE concentration of after 24 hr incubation. 6. A significant increase in the production of (TNF)- was detected with ACE concentrations of 50, 100, and after 24 hr incubation.
Conclusions: These show that ACE increases mouse macrophage NO production at concentrations above , and the cytokines ((IL)-, IL-6, and (TNF)-) at concentrations above . These results suggest that ACE improves macrophage immune property.
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KEYWORD
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Angelicae pubescentis radix, cytokine, immune property, macrophage, nitric oxide
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